The C-Terminal Flanking Peptide of NPY is transiently upregulated in DRG following tourniquet compression
Field:	Other
Authors:	Widerberg, Annika Kanje, Martin Dahlin, Lars B
Address of presenting author:	Department of Hand Surgery, Malmö University Hospital, 205 02 Malmö
E-mail:	annika.widerberg@hand.mas.lu.se
Phone:	+4640-336769
Fax:	+4640-925588
Text of abstract:	The C-flanking terminal of Neuropeptide Y (CPON) is normally not expressed in uninjured sensory nerve cell bodies in dorsal root ganglia (DRG), but following extended nerve lesions, like transection of a peripheral nerve, a significant upregulation of CPON can be seen. We investigated if a minor trauma as acute compression of the rat sciatic nerve could induce upregulation of CPON in DRG. Compression of the rat sciatic nerve was induced by application of a mini-tourniquet around the hind limb at midthigh level for two hours at 150 or 300 mmHg. The expression of CPON in DRG was studied by immunocytochemistry after 3-28 days of recovery and was related to the expression of CPON following sciatic nerve transection. There was no expression of CPON in control DRG at any timepoints. Transection caused a continuous increasing upregulation of CPON in up to 20% of the cells after 6 days and 30 % after four weeks, while tourniquet compression at 300 mmHg induced a transient expression of CPON in up to 3 and 5 % of the cells after 3 and 6 days, respectively. A magnitude of 150 mm Hg caused expression of CPON in a lesser number of cells. Tourniquet compression can induce a transient upregulation of CPON in the DRG which is, however, not of the same extent as seen after permanent nerve transection. The present data indicates that peripheral nerve compression affects the whole neuron and that NPY may have a pain modulating effect.
Keywords:	Neuropeptide Y, NPY, C-terminal flanking peptide of Neuropeptide Y, CPON, nerve compression

Created 2000-04-05