Studies on the Expression of the Phospholipase D1 and D2 Genes in the Central Nervous System of the Adult Rat.

Studies on the Expression of the Phospholipase D1 and D2 Genes in the Central Nervous System of the Adult Rat.

Field: Disorders of the nervous system

Authors: Aradóttir, Steina
Kanje, Martin
Alling, Christer
Moller, Kristian.

Address of presenting
author: Inst. för laboratoriemedicin
avd. för medicinsk neurokemi
Universitetssjukhuset i Lund
221 85 Lund

E-mail: steina.aradottir@neurokemi.lu.se

Phone: 046-175374

Fax: 046-149870

Text of abstract: The mammalian phospholipase D (PLD) constitute a family of enzymes whose activation trigger a wide variety of signal transduction events. During ethanol-treatment this enzyme catalyses the formation of phosphatidylethanol (PEth). PEth being an unphysiological phospholipid might be responsible for some of the organ damages caused by ethanol.
The recent cloning of mammalian PLD1 and PLD2 cDNAs has made it possible to localize these messages by in situ hybridization (ISH) histochemistry.
The recent findings of isoenzymes raise questions on a possible correlation between the different types of PLD enzymes and the formation of PEth in major organs of the body as well as such correlation at a cellular level. Is there a correlation between the different types of PLD enzymes in a certain tissue and the formation of PEth in that tissue ?
We have used [³⁵S] labelled cRNA probes specific for rat PLD1 and PLD2 to localize these mRNAs in sections of rat brain. PLD1 mRNA was extremely rare and difficult to detect. PLD2 mRNA was more abundant and the most intense autoradiographic signal was seen in choroid plexus. PLD2 mRNA was also found in scattered cells in gray matter. Certain hippocampal neurons showed a notably high expression

Keywords: Phospholipase D (PLD), rat, mRNA, in situ hybridization

	Studies on the Expression of the Phospholipase D1 and D2 Genes in the Central Nervous System of the Adult Rat.
Field:	Disorders of the nervous system
Authors:	Aradóttir, Steina Kanje, Martin Alling, Christer Moller, Kristian.
Address of presenting author:	Inst. för laboratoriemedicin avd. för medicinsk neurokemi Universitetssjukhuset i Lund 221 85 Lund
E-mail:	steina.aradottir@neurokemi.lu.se
Phone:	046-175374
Fax:	046-149870
Text of abstract:	The mammalian phospholipase D (PLD) constitute a family of enzymes whose activation trigger a wide variety of signal transduction events. During ethanol-treatment this enzyme catalyses the formation of phosphatidylethanol (PEth). PEth being an unphysiological phospholipid might be responsible for some of the organ damages caused by ethanol. The recent cloning of mammalian PLD1 and PLD2 cDNAs has made it possible to localize these messages by in situ hybridization (ISH) histochemistry. The recent findings of isoenzymes raise questions on a possible correlation between the different types of PLD enzymes and the formation of PEth in major organs of the body as well as such correlation at a cellular level. Is there a correlation between the different types of PLD enzymes in a certain tissue and the formation of PEth in that tissue ? We have used [³⁵S] labelled cRNA probes specific for rat PLD1 and PLD2 to localize these mRNAs in sections of rat brain. PLD1 mRNA was extremely rare and difficult to detect. PLD2 mRNA was more abundant and the most intense autoradiographic signal was seen in choroid plexus. PLD2 mRNA was also found in scattered cells in gray matter. Certain hippocampal neurons showed a notably high expression

Keywords:	Phospholipase D (PLD), rat, mRNA, in situ hybridization

Index

Created 2000-04-12

Department of Physiological Sciences, Lund University

Web programming FormOnLine AB